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CABRI:ICLC |
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Accession_number |
ICLC ATL98012
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Cell_line_name |
A8/D1
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Brief_description |
Species: mouse, AKR; Tissue: thymus; Tumor: thymoma; exposed to a dose of 500 rads from an X-ray source; Original line: AKR/1a murine T cell line
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Description |
Species: mouse, AKR; Tissue: thymus; Tumor: thymoma; the mutant Lyt-2 cells were selected out by magnetic bead separation; Original line: AKR/1a murine T cell line
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Depositor |
Obtained from ECACC
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Bibliographic_reference |
J Immunol Methods 1992;154(2):211-216 [PMID: 1401954
] |
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Morphology |
lymphoblast, grown in suspension
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Culture_conditions |
continuous culture; DMEM + 10% HS + 2mM L-Glutamine + 1mM Na pyruvate; maintain cultures between 3-9x10^5 cells/ml; split 1:5-1:10; 37C, 5% CO2
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Viruses |
Search for viruses was not performed
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Properties |
useful in the study of assembly and transport of Lyt-2/3 antigen in the cell surface or to define the regions of Lyt-2/3 interacting with the corresponding antibodies and with MHC class I molecules
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Release_conditions |
Cell line available for distribution. For non-commercial investigative use only
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Hazard |
-
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Species_validation |
Validated by isoenzymes: confirmed as mouse with AST, MD
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Freezing_medium |
Culture medium + 50% FBS + 10% DMSO
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Sterility |
mycoplasma negative, HOECHST and PCR
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Further_bibliography |
Immunogenetics 1994;40(2):154-158 [PMID: 7517914
]
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